PREreview of Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography

This is an excellent experimental setup and I read the manuscript with great interest. My major question has to do with the modeling in Figure 2. Is there no solvent in any of the substrate/product states or an apo state that needs to be modeled in the active site. This seems like it might have a major effect on the estimated occupancies.

Competing interests

The author declares that they have no competing interests.