The increasing adoption of high-throughput "omics" technologies has heightened the demand for standardized, scalable, and reproducible bioinformatics workflows. Nextflow and nf-core provide a robust framework for researchers, particularly early- and mid-career researchers (EMCRs), to navigate complex data analysis. At The Kids Research Institute Australia, we implemented a structured approach to bioinformatics capacity building using these tools. This paper presents ten practical rules that facilitated the successful adoption of Nextflow and nf-core, addressing implementation, knowledge gaps, resource allocation, and community support. Our experience serves as a guide for institutions aiming to establish sustainable bioinformatics capabilities and empower EMCRs.