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Distinct Working Memory for Near and Far in a T-Maze Delayed Alternation Task

Publié
Serveur de preprints
bioRxiv
DOI
10.64898/2026.07.30.741937

Working memory has been considered a delay-dependent retention system, but variability in delay duration across species and tasks suggests that task-intrinsic factors may also contribute. Here, we investigated the effects of delay duration (75 s vs. 150 s) and task distance (0 m vs. 2 m) on working memory using a T-maze delayed alternation task with a movable home-cage apparatus in rats. At the 150-s delay, accuracy was 9% lower at 0 m than at 2 m (75% vs. 84%), with a similar numerical tendency observed at the 75-s delay (4% lower). A two-way repeated measures ANOVA revealed significant main effects of both task distance and delay duration, with no significant interaction (p = 0.217). Post hoc pairwise comparisons indicated that accuracy in the 0-m condition was significantly lower at 150 s than at 75 s (adjusted p = 0.038). At the 150-s delay, accuracy was also significantly lower in the 0-m condition than in the 2-m condition (adjusted p = 0.019). These results suggest that working memory retention is influenced not only by temporal constraints but also by task-intrinsic factors such as task distance, with the observed effects appearing independent and consistent with a two-factor framework.

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