Okra (Abelmoschus esculentus) stems constitute an abundant lignocellulosic biomass with significant potential for sustainable composite reinforcement. In this study, okra fibers were extracted using biological retting, alkaline treatment (1-7.5 wt% NaOH), and combined extraction processes. The influence of extraction conditions on the physicochemical, mechanical, thermal, and structural properties of the fibers was investigated. FTIR analysis revealed the progressive removal of hemicellulose and lignin after alkaline treatment, while XRD results showed an increase in cellulose crystallinity. Optical microscopy observations revealed progressive fiber separation and cleaner surface morphology after alkaline treatment. Fiber density increased with NaOH concentration, whereas water absorption and moisture regain decreased due to the reduction of hydrophilic amorphous components. Mechanical properties, particularly tensile strength and Young’s modulus, improved under moderate treatment conditions but decreased under severe alkaline conditions because of partial cellulose degradation. The optimal treatment condition (1 wt% NaOH for 60 min) provided the best balance between delignification, structural preservation, and mechanical performance. These results demonstrate that okra fibers are promising lightweight reinforcements for sustainable bio-composite and technical textile applications.