A novel flavi–like virus such as Alongshan virus (ALSV) with segmented genomes have been early isolated from Ixodes ticks in Russia. In this study, the ALSV genetic markers were tested in 4458 ixodid ticks collected in 22 regions of European and Asian part of Russia by RT PCR. The highest infection rate of ALSV in ticks was detected for Khakassia Republic (3.3%) and Kemerovo region (2.4%) and low infection rate was more typical for European part of Russia (0.4–0.7%). The sequencing complete genomes (four segments) of the ALSV from 22 PCR positive Ixodes persulcatus ticks was carry out by high–throughput sequencing. The identity level nucleotide sequences for Asian ALSV isolates are 94.5–96.5% with ALSV isolates previously found in China and in 89–93% range for the European isolates. This data together with phylogenetic analysis are propose the existence of Asian and European subtypes of the ALSV, and they may be associated with I. persulcatus and I ricinus ticks. The obtained results actualize spreading ALSV in Russia and also may be useful for diagnostics, prophylactic and treatment this infection.