Avaliações PREreview de “Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis”

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Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis

de Dominik Trzupek, Melanie Dunstan, Antony J. Cutler, Mercede Lee, Leila Godfrey, Dominik Aschenbrenner, Holm H. Uhlig, Linda S. Wicker, John A. Todd e Ricardo C. Ferreira

Publicado: 18 de julho de 2019
Servidor: bioRxiv
DOI: 10.1101/706275

Resumo

The transcriptomic and proteomic characterisation of CD4⁺T cells at the single-cell level has been performed traditionally by two largely exclusive types of technologies: single cell RNA-sequencing (scRNA-seq) technologies and antibody-based cytometry. Here we demonstrate that the simultaneous targeted quantification of mRNA and protein expression in single-cells provides a high-resolution map of human primary CD4⁺T cells, and identified precise trajectories of Th1, Th17 and regulatory T-cell (Treg) differentiation in blood and tissue. Furthermore, the sensitivity provided by this massively-parallel multi-omics approach revealed novel insight into the mechanism of expression of CD80 and CD86 on the surface of activated CD4⁺Tregs and demonstrate their potential to identify recently activated T cells in circulation. This transcriptomic and proteomic hybrid technology provides a cost-effective solution to dissect the heterogeneity of immune cell populations, including more precise and detailed descriptions of the differentiation and activation of circulating and tissue-resident cells in response to therapies and in stratification of patients.

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Avaliação PREreview de Hector Hernandez-Vargas

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PREreview of "Simultaneous mRNA and protein quantification at the single-cell level delineates trajectories of CD4+ T-cell differentiation"
Journal Club PREreview by: Hector Hernandez-Vargas, Apostol Apostolov, Olivier Fesneau, Ossama Labiad, Valentin Thevin, Vincent Flacher, Saidi Soudja, Julien…

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Avaliações PREreview de Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis

1 Avaliação PREreview

PREreview of "Simultaneous mRNA and protein quantification at the single-cell level delineates trajectories of CD4+ T-cell differentiation"