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Development of an immunoassay for the detection and diagnosis of microbially-influenced corrosion caused by methanogenic Archaea

de Sven Lahme e Jorge Mantilla Aguas

Publicado: 8 de agosto de 2024
Servidor: bioRxiv
DOI: 10.1101/2024.08.08.607177

Resumo

Microbially-influenced corrosion (MIC) is a costly problem across several industries. The steadily rising use of advanced molecular biological methods to investigate MIC allowed ever deeper insights in the underlying microbial community structure and function. However, currently available technologies do not allow accurate detection and diagnosis of MIC in the field. Recent discovery of a special hydrogenase in certain corrosive methanogenic Archaea allowed for the development of a first ever MIC biomarker termed heremicH. ThemicHgene encodes the large subunit of a special [NiFe] hydrogenase involved in MIC. Here we describe the development of a recombinant antibody that enable the specific and sensitive detection of the MicH protein in western blot immunoassays. Using a recombinant MicH protein we determined the lower limit of detection per assay to be around 0.3 fg MicH. The immunoassay was able to detect a strong signal for the MicH protein inmicH-positive pure cultures ofMethanobacterium-like. strain IM1 that was cultivated on iron granules, and the signal was over 500 times lower in amicH-negativeMethanococcus maripaludisS2 culture. To further evaluate the ability to differentiate corrosive from non-corrosive microbial communities, we tested ten oil field enrichment cultures that showed a wide range of corrosion rates (0.02 – 0.48 mm/yr). We detected the MicH protein in planktonic (36.5 – 1473.5 pg/mL) and carbon steel biofilm samples (41.0 – 7971.3 pg/cm²) from corrosive methanogenic enrichments (0.17 – 0.48 mm/yr) but did not detect MicH in any of the non-corrosive tests (<0.08 mm/yr) despite methanogenic activity. The results indicate that corrosion was likely caused by methanogenic Archaea expressing a corrosive [NiFe] hydrogenase and that the newly developed MicH-specific immunoassay can detect and monitor their activity. The development of a specific and sensitive immunoassays to detect a MIC biomarker allow corrosion scientists and field practitioners to detect and monitor the activity of corrosive methanogenic Archaea.

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Avaliação PREreview feita por Femi Qudus Arogundade et al. do/a ASAPbio Microbiology Crowd

De autoria de Femi Qudus Arogundade, Ahmad Bashiru Inuwa, Emmanuel Adamolekun, Randa Salah Gomaa Mahmoud, Teena Bajaj, Chen Yang, Gliday Yuka e 1 outro autor do/a ASAPbio Microbiology Crowd

This review incorporates comments and contributions from Femi Arogundade, Aneth David, Chen Yang, Teena Bajaj, Ahmad Bashiru, Randa Salah Gomaa Mahmoud, Gliday Yuka, and Adamolekun Emmanuel. The review was synthesized by Femi Arogundade.
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Avaliações PREreview de Development of an immunoassay for the detection and diagnosis of microbially-influenced corrosion caused by methanogenic Archaea

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