Avaliações PREreview de Using AlphaFold Multimer to discover interkingdom protein-protein interactions

Ir para detalhes do preprint Ir para avaliações PREreview

Using AlphaFold Multimer to discover interkingdom protein-protein interactions

de Felix Homma, Joy Lyu e Renier A. L. van der Hoorn

Publicado: 14 de junho de 2024
Servidor: bioRxiv
DOI: 10.1101/2024.06.14.599045

Resumo

Structural prediction by artificial intelligence (AI) can be powerful new instruments to discover novel protein-protein interactions, but the community still grapples with the implementation, opportunities and limitations. Here, we discuss and re-analyse our in-silico screen for novel pathogen-secreted inhibitors of immune hydrolases to illustrate the power and limitations of structural predictions. We discuss strategies of curating sequences, including controls, and reusing sequence alignments and highlight important limitations originating from platforms, sequence depth and computing times. We hope these experiences will support similar interactomic screens by the research community.

Ler o preprint

1 PREreview

Escrever uma Avaliação PREreview Solicitar uma Avaliação PREreview

Avaliação PREreview feita por Andrés Posbeyikian do/a TSL Preprint Club

De autoria de Andrés Posbeyikian do/a TSL Preprint Club

Preprint review of “Using AlphaFold Multimer to discover interkingdom protein-protein interactions”

By Andrés Posbeyikian
…
Ler a avaliação PREreview de Andrés Posbeyikian do/a TSL Preprint Club

Avaliações PREreview de Using AlphaFold Multimer to discover interkingdom protein-protein interactions

1 PREreview

Preprint review of “Using AlphaFold Multimer to discover interkingdom protein-protein interactions”