Ir para o conteúdo principal

Escrever uma avaliação PREreview

Targeted phage hunting to specificKlebsiella pneumoniaeclinical isolates is an efficient antibiotic resistance and infection control strategy

Publicado
Servidor
bioRxiv
DOI
10.1101/2024.01.07.574526

Klebsiella pneumoniaeis one of the most threatening multi-drug resistant pathogens today, with phage therapy being a promising alternative for personalized treatments. However, the intrinsic capsule diversity inKlebsiellaspp. poses a substantial barrier to phage host range, complicating the development of broad-spectrum phage-based treatments. Here, we have isolated and genomically characterized phages capable of infecting each of the acquired 77 reference serotypes ofKlebsiellaspp.,including capsular types widespread among high-riskK. pneumoniaeclones causing nosocomial infections. We demonstrated the possibility of isolating phages for all capsular types in the collection, revealing high capsular specificity among taxonomically related phages, in contrast to a few phages that exhibited broad-spectrum infection capabilities. To decipher the determinants of the specificity of these phages, we focused on their receptor-binding proteins, with particular attention to depolymerase domains. We also explored the possibility of designing a broad-spectrum phage cocktail based on phages isolated in reference capsular type strains, and determining the ability to lysate relevant clinical isolates. Interestingly, a combination of 12 phages capable of infecting 60% of the referenceKlebsiellaspp. serotypes was tested on a panel of carbapenem-resistantK. pneumoniaeclinical isolates. Our results suggest that in a highly variable encapsulated bacterial host, phage hunting must be directed to the specificKlebsiellaisolates. This work is a step forward in the understanding of the complexity of phage-host interactions, and highlights the importance of implementing precise and phage-specific strategies to treatK. pneumoniaeinfections worldwide.

Você pode escrever uma avaliação PREreview de Targeted phage hunting to specificKlebsiella pneumoniaeclinical isolates is an efficient antibiotic resistance and infection control strategy. Uma avaliação PREreview é uma avaliação de um preprint e pode variar de algumas frases a um parecer extenso, semelhante a um parecer de revisão por pares realizado por periódicos.

Antes de começar

Vamos pedir que você faça login com seu ORCID iD. Se você não tiver um iD, pode criar um.

O que é um ORCID iD?

Um ORCID iD é um identificador único que diferencia você de outras pessoas com o mesmo nome ou nome semelhante.

Começar agora