Avaliações PREreview de “Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic Wounds”

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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic Wounds

de Heema K. N. Vyas, Binbin Xia, David Alam, Nicholas P. Gracie, Joanna G. Rothwell, Scott A. Rice, Dee Carter, Patrick J. Cullen e Anne Mai-Prochnow

Publicado: 27 de julho de 2023
Servidor: bioRxiv
DOI: 10.1101/2023.07.26.550769

Resumo

Healing and treatment of chronic wounds are often complicated due to biofilm formation by pathogens. Here, the efficacy of Plasma Activated Water (PAW) as a pre-treatment strategy has been investigated prior to the application of topical antiseptics polyhexamethylene biguanide, povidone iodine, and MediHoney, which are routinely used to treat chronic wounds. The efficacy of this treatment strategy was determined against biofilms ofEscherichia coliformed on a plastic substratum and on a human keratinocyte monolayer substratum used as anin vitrobiofilm-skin epithelial cell model. PAW pre-treatment greatly increased the killing efficacy of all the three antiseptics to eradicate theE. colibiofilms formed on the plastic and keratinocyte substrates. However, the efficacy of the combined PAW-antiseptic treatment and single treatments using PAW or antiseptic alone was lower for biofilms formed in thein vitrobiofilm-skin epithelial cell model compared to the plastic substratum. Scavenging assays demonstrated that reactive species present within the PAW were largely responsible for its anti-biofilm activity. PAW treatment resulted in significant intracellular RONS accumulation within theE. colibiofilms, while also rapidly acting on the microbial membrane leading to outer membrane permeabilisation and depolarisation. Together, these factors contribute to significant cell death, potentiating the antibacterial effect of the assessed antiseptics.

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Avaliação PREreview feita por Liu Xuyu et al. do/a SG Biofilms and Microbiome Club

De autoria de Liu Xuyu, Clarence Sim, Hashmath Fatimah, Samantha Quah, Sreelakshmi Cheruvalli, Hana Marican e Viduthalai Rasheedkhan Regina do/a SG Biofilms and Microbiome Club

Reviewed by: Liu Xuyu, Clarence Sim, Hashmath Fatimah, Samantha Quah, Sreelakshmi Cheruvalli, Hana Marican and Viduthalai Rasheedkhan Regina
Singapore Centre for Environmental Life Science Engineering, Nanyang Technological University, Singapore
Summary:
The paper discusses plasma activated water…

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Avaliações PREreview de Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic Wounds

1 Avaliação PREreview