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The Magnaporthe oryzae Effector AvrPii Attenuates Rice Immunity by Targeting the Calcium-Associated ANK Protein AVIN8

par Wei Yao, Fuhui Yang, Xiaolong Zhou, Qianru Chen, Jinjun Lin, Yuying Zou, Chenyang Xu, Bei He, Dandan Zhu, Sisi Ji, Xionglun Liu et Jinling Liu

Publié: 16 mai 2026
Serveur de preprints: bioRxiv
DOI: 10.64898/2026.05.13.725024

Résumé

Rice blast, caused by the fungus Magnaporthe oryzae , poses a severe threat to global rice production. Although numerous M. oryzae effectors have been identified, the molecular mechanisms remain poorly understood. The effector AvrPii triggers ETI upon recognition by NLR receptor Pii and has been shown to target host exocytosis and oxidative metabolic pathways to suppress immunity. Here, we identify AVIN8 ( Av rPii- in teracting protein 8) as a new target of AvrPii. AVIN8 is an ankyrin-repeat (ANK) protein localized to the plasma membrane and nuclear envelope, with sequence similarity to known ANK calcium channels. AVIN8 expression is induced during early M. oryzae infection. Overexpression of AVIN8 enhances blast resistance, whereas its silencing increases susceptibility. AVIN8 promotes chitin-triggered ROS production in a Ca ²⁺ -dependent manner. In contrast, AvrPii impairs Ca ²⁺ influx and chitin-triggered ROS burst. Our findings reveal a virulence strategy in which M. oryzae effector AvrPii hijacks the Ca ²⁺ influx-associated ANK protein AVIN8 to suppress early calcium and PTI signaling in rice.

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PREreview Sophien Kamoun et al. du club TSL Preprint Club

Rédigé par Sophien Kamoun et Matteo GRAVINO du TSL Preprint Club

Figure 1A: Yeast Two-Hybrid

Figure 1A is presented as the first evidence that AvrPii interacts with AVIN8. The evidence is not convincing. The yeast two-hybrid assay lacks the controls required to distinguish a specific interaction from a false-positive signal caused by a sticky bait or prey, and…

Lire la PREreview de Sophien Kamoun et d’autres membres du club TSL Preprint Club

PREreviews de The Magnaporthe oryzae Effector AvrPii Attenuates Rice Immunity by Targeting the Calcium-Associated ANK Protein AVIN8

1 PREreview

Figure 1A: Yeast Two-Hybrid