In the underground operation scenario, with the development of intelligence, the underground electronic monitoring and control systems have gradually become an important tool for mining practitioners to obtain operational information. In view of the existence of low-light environment under the mine and the obvious difference from the above-ground natural light, screen-related factors have a significant impact on the visual search task, so it is critical to study the impact of interface layout in the low-light environment under the mine on the visual search efficiency. In this study, the application scenario of the electronic monitoring and control system under the mine is simulated. The information layout of the current electronic monitoring and control system in the mine and different lighting environments are set as experimental variables. The effects of interface layout design features on user search performance, visual behavior and usability satisfaction are discussed. The experimental results show that interface layout and illumination change have significant main effects on task completion time, fixation times, saccade ratio and subjective usability score. Among them, Three-Column layout mode has outstanding performance in the aspects of task completion time, fixation number and subjective usability score, and the search efficiency is higher in 50lx illumination environment.