Zirconia is known as a strong and bioinert load bearing material for dental implants. It usually exhibits no antibacterial activity. Inflammation is a crucial problem for dental implant surgery. About 3-5% of all dental implants experience inflammation. It is very central finding of the present study that fullerene C60 films as well as a tribomechanical loading of zirconia without the fullerene C60 topping can cause an improvement of antibacterial activity against gram-positive Staphylococcus aureus. The moderate antibacterial activity is especially important, because a strong antibacterial effect could disturb the sensitive oral bacterial flora and should be prevented. In the present study, different conditions of the fullerene C60 film were provided. In addition to fullerene C60 film in the “as deposited” condition, treatment in a nitrogen plasma as well as tribomechanical produced surface pattern without and with plasma post-treatment were testified. 85.8% reduction of gram-positive Staphylococcus aureus bacterial formation was measured on zirconia with fullerene C60 film. Plasma treatment of the C60 film effect an increase of the antibacterial impact of 72.2% only in comparison to zirconia without fullerene C60 film. Also, tribomechanical loaded fullerene C60 films suppress the growth of gram-positive Staphylococcus aureus. The tribomechanical loading seems to compensate the effect of a plasma treatment. ZrO2 samples with fullerene C60 film and tribomechanical loading achieve an increase of antibacterial impact of 83.36%. Furthermore, surprisingly yttria-stabilized zirconia bioceramic without fullerene C60 film also shows a high antibacterial effectivity after a tribomechanical patterning procedure. Due to surficial patterning the ZrO2 by scratching microgroove arrangements with a diamond tip, its antibacterial effect against gram-positive Staphylococcus aureus was increased 70.46%.