Global biodiversity is declining at an alarming rate; however, evidence suggests that this decline occurs far more slowly on Indigenous-owned land. This can be attributed to cultural worldviews in which protecting nature and living in harmony with the environment are fundamental principles, an ethos central to African societies and many other Indigenous communities worldwide. This study examines the role of Vhavenda traditional belief systems, Indigenous knowledge, and cultural practices in the management and conservation of natural resources and the environment. In contemporary Limpopo Province, the Vhavenda clans of northern South Africa remain among the country’s most traditional communities, continuing rituals and practices that have been transmitted across generations. According to the 2022 national census, the area inhabited by the Vhavenda tribe, records the country’s highest concentration of centenarians, a demographic pattern which they attribute to the region’s cultural continuity and relative geographical isolation, which have enabled the preservation of its spiritual and ecological heritage. The research employed an insider ethnographic methodology, collecting data through personal interviews and a focus group discussion. Findings reveal that Indigenous beliefs, knowledge systems, and taboos play a substantial role in promoting sustainable land use. They restrict development on ecologically sensitive landscapes and discourage harmful practices, such as deforestation and cultivation along water bodies. These practices are enforced through complex customary laws, often articulated through prohibitive norms (“thou shalt not”), that safeguard plants, animals, water sources, and other natural resources. The study further illustrates that these prohibitions reflect a nuanced understanding of the biophysical environment, with the most sensitive and vulnerable ecosystems and ecologically important species, including keystone, foundation, and indicator species, receiving protection. Overall, the research shows the importance of recognising, protecting, and integrating Indigenous cultural systems as a critical component of effective biodiversity conservation.