Amazonian large trees act as central elements of forest ecosystems, storing a disproportionate fraction of aboveground biomass. However, these trees are not randomly distributed across the landscape, and it is expected that edaphic attributes influence floristic composition, forest structure, and vegetation biomass. In this study, we investigated how soil variation affects the diversity and biomass of large trees. Forest inventories were conducted at five sites within protected areas in the states of Pará and Amapá. Aboveground biomass was estimated using allometric equations, while soil samples were analyzed for their physical and chemical properties. Diversity indices, rarefaction, Redundancy Analysis, and Generalized Additive Models were applied. Edaphic variables such as soil pH, organic matter, phosphorus, and aluminum were associated with floristic composition and the biomass of these individuals. Trees with a diameter at breast height greater than or equal to 70 centimeters accounted for up to 80% of total biomass, revealing a pattern of biomass hyperdominance. The results indicate that the occurrence of large trees is related to edaphic and structural factors, suggesting that these individuals are not randomly distributed along soil gradients. Understanding these patterns is essential for improving ecological models, biomass extrapolations, and management strategies aimed at conserving the Amazon rainforest.