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Background: Inertial measurement units (IMUs) represent a relatively new and promising method for motion analysis. Their main advantages include small size and portability, combined with the use of advanced technologies. To date, few studies have investigated the application of these devices for proprioception assessment, and none have focused specifically on the elbow joint. Therefore, the aim of our study was to assess reliability and validate the protocol of elbow proprioception evaluation using inertial motion sensors. Methods: Twenty healthy participants underwent active and passive proprioception assessments based on joint position sense (JPS). Two researchers independently performed evaluation. Analyzed data was error of reproduction of joint position (ERJP). IMU (RSQ Motion sensors) were used for angular joint position assessment and validated against Biodex System 4. Results: Inter-rater reliability for passive proprioception was good, with a Kendall’s coefficient of 0.77 (p < 0.05) for both RSQ Motion sensors and BIODEX, while active proprioception measured with RSQ Motion sensors showed slightly lower reliability (Kendall’s coefficient of 0.66, p < 0.05). Intra-rater reliability had similar results, with Kendall’s coefficients of 0.74 for passive BIODEX proprioception examination, 0.75 for passive RSQ Motion sensor testing and 0.65 for active proprioception (p < 0.05) measured with RSQ Motion sensors. Bland-Altman plot revealed equal distribution of results, which were within the limits of agreement (LoA). Conclusion: These results suggest proprioception assessment by JPS using inertial motion sensors is reliable and valid. Easy to use, light, portable, and inexpensive alternative for proprioception assessment, although further research in diverse clinical settings is needed.