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Gel-based coupling agents are particularly favored for their long-term stability, high coupling efficiency, and ease of preparation, making them integral to acoustic flow control applications. In practice, coupling agents facilitate microparticle trapping, droplet manipulation, and biosample sorting through acoustic impedance matching and wave mode conversion (e.g., Rayleigh-to-Lamb waves). Their thickness and acoustic properties (sound velocity, attenuation coefficient) further modulate sound field distribution to optimize acoustic radiation forces and thermal effects. However, challenges remain regarding stability (evaporation, thermal degradation) and chip compatibility. Further aspects of research into gel-based agents requiring attention include multilayer coupled designs, dynamic thickness control, and enhancing biocompatibility to advance acoustofluidic technologies in point-of-care diagnostics and high-throughput analysis.