PREreviews de Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis

Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis

par Valerie F. A. March, Nino Maghradze, Kakha Mchedlishvili, Teona Avaliani, Rusudan Aspindzelashvili, Zaza Avaliani, Maia Kipiani, Nestani Tukvadze, Levan Jugheli, Selim Bouaouina, Anna Doetsch, Galo A. Goig, Sebastien Gagneux et Sonia Borrell

Publié: 5 août 2024
Serveur de preprints: bioRxiv
DOI: 10.1101/2024.08.05.606579

Résumé

Differential culturable bacteria grow in liquid culture medium but are unable to form colonies on solid medium. Differentially culturable Mycobacterium tuberculosis (Mtb) bacteria, have been found in tuberculosis (TB) patient sputa. We hypothesized that antibiotic treatment can induce differential culturability in Mtb. We investigated the effect of exposure to TB drugs on Mtb culturability using clinical samples from an ongoing TB patient cohort and by conducting several in vitro experiments with a diverse set of Mtb strains. In patients, serial sputa were more likely to generate Mtb-positive cultures in liquid as opposed to solid medium, with this liquid culture bias extending up to 5 months post diagnosis. Experimentally, there was a disparity between bacterial time to positivity (TTP) and colony forming units (CFUs) when Mtb was exposed to isoniazid (INH) and rifampicin (RIF) alone or in combination. Cultures recovered from RIF treatment yielded more CFUs on agar plates, but INH-treated cultures had a faster TTP in liquid. Follow up experiments using a fluorescently labelled laboratory strain of Mtb revealed that CFUs overestimated killing by INH treatment. Here we provide evidence in Mtb that drug exposure affects culturability on solid medium, which has implications for treatment monitoring and drug-pathogen interaction studies.

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PREreview Corrado Nai et al. du club ASAPbio Microbiology Crowd

Rédigé par Corrado Nai, Oluwaseun Adeolu Ogundijo, Gliday Yuka, Musa Ali, Teena Bajaj, Vaishnavi Nagesh et 1 autre auteur du ASAPbio Microbiology Crowd

Review of the preprint “Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis”

Authors: Valerie F. A. March, Nino Maghradze, Kakha Mchedlishvili, Teona Avaliani, Rusudan Aspindzelashvili, Zaza Avaliani, Maia Kipiani, Nestani Tukvadze, Levan Jugheli, Selim…

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PREreview par Corrado Nai et al.

Rédigé par Corrado Nai et 6 autres auteurs

Review of the preprint “Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis”

Authors: Valerie F. A. March, Nino Maghradze, Kakha Mchedlishvili, Teona Avaliani, Rusudan Aspindzelashvili, Zaza Avaliani, Maia Kipiani, Nestani Tukvadze, Levan Jugheli, Selim…

Lire la PREreview de Corrado Nai et al.

PREreviews de Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis

2 PREreviews

Review of the preprint “Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis”

Review of the preprint “Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis”