PREreviews de Circulating tumor extracellular vesicles to monitor metastatic prostate cancer genomics and transcriptomic evolution

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Circulating tumor extracellular vesicles to monitor metastatic prostate cancer genomics and transcriptomic evolution

par Irene Casanova-Salas, Sarai Cordoba-Terreros, Daniel Aguilar, Laura Agundez, Julian Brandariz, Nicolas Herranz, Macarena Gonzalez, Rafael Morales-Barrera, Alexandre Sierra, Mario Soriano-Navarro, Pablo Cresta, Sara Simonetti, Gonçalo Rodrigues, Sara Arce-Gallego, Luisa Delgado-Soriano, Irene Agustí, Elena Castellano-Sanz, Richard Mast, Matias de Albert, Ana Celma, Anna Santamaria, Lucila Gonzalez, Natalia Castro, Maria del Mar Suanes, Javier Hernández-Losa, Lara Nonell, Hector Peinado, Joan Carles et Joaquin Mateo

Publié: 14 avril 2023
Serveur de preprints: bioRxiv
DOI: 10.1101/2023.04.14.536404

Résumé

Extracellular vesicles (EVs) secreted by tumors are abundant in plasma, but their potential for interrogating the molecular features of tumors through multi-omic profiling remains widely unexplored. Genomic and transcriptomic profiling of circulating EV-DNA and EV-RNA isolated from a range of in-vitro and in-vivo models of metastatic prostate cancer (mPC) revealed a high contribution of tumor material to EV-loaded DNA/RNA. Findings were validated in a cohort of longitudinal plasma samples collected from mPC patients during androgen receptor signaling inhibitor (ARSI) therapy. EV-DNA genomic features recapitulated matched-patient biopsies and associated with clinical progression. We developed a novel approach to enable the transcriptomic profiling of EV-RNA (RExCuE). We report how the transcriptomic profile in mPC EV-RNA is enriched for tumor-associated transcripts when compared to same patient blood RNA and healthy individuals EV-RNA, and reflect early on-therapy tumor adaptation changes. Altogether, we show that EV profiling enables longitudinal transcriptomic and genomic profiling of mPC in liquid biopsy.

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PREreview Isaac Arddey et al. du club ASAPbio Cancer Biology Crowd

Rédigé par Isaac Arddey, Teena Bajaj, Sabareeswaran Krishnan, Arpita Ghosh, Akanksha Verma, Rebecca A Shelley, Ghada Tagorti, Garima Jain, Harini Lakshminarayanan, Adriana Mena et Ruchika Bajaj du ASAPbio Cancer Biology Crowd

This review reflects comments and contributions from Arddey Isaac, Teena Bajaj, Sabareeswaran Krishnan, Arpita Ghosh, Akanksha, Rebecca A Shelley, Ghada Tagorti, Garima Jain, Harini Lakshminarayanan, Adriana Mena & Ruchika Bajaj. Review synthesized by Garima Jain & Arpita Ghosh

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