PREreview del FOXA2 potently represses viral gene expression by targeting NF-κB–dependent transcription in liver cells

Write a short summary of the research’s main findings and how this work has moved the field forward.

Major issues:

• ATAC-seq: The study would benefit from clearer evidence showing that enhancers are closed in wild-type (WT) conditions and become accessible primarily in FOXA2^low contexts, to support conclusions about chromatin accessibility dynamics.

• CUT&Tag: It is important to further clarify whether enhancer occupancy by transcription factors is restricted to FOXA2-deficient conditions, and how FOXA2 presence, even at low levels, contributes to enhancer repression.

• CRISPR deletion: Functional deletion of candidate enhancers suggests enhancer-specific regulation; however, additional validation across multiple loci would strengthen the generalizability of enhancer-specific control.

Minor issues:

• The study appears to rely heavily on Western blot analyses, which may not be sufficient on their own to fully support conclusions related to chromatin state and transcriptional regulation.

• Control conditions are not always explicitly demonstrated or clearly described across experiments, which may limit the interpretation of causality in some of the reported findings.

Competing interests

The author declares that they have no competing interests.

Use of Artificial Intelligence (AI)

The author declares that they used generative AI to come up with new ideas for their review.