Acromegaly is an endocrine disorder predominantly resulting from a benign pituitary adenoma that causes excessive growth hormone secretion. The current study was designed to investigate the percentage of the 95A>T polymorphism in acromegaly patients. This study was conducted on 80 whole blood samples from acromegaly patients, which were collected from Al-Mustansiriyah University, Specialist Center for Diabetic National, and 40 samples from healthy controls. a targeted region of the GHRH gene was amplified via conventional polymerase chain reaction (PCR) in all samples. Sequence analysis revealed a novel single nucleotide polymorphism (SNP), 95A>T, which was detected in 67.5% (54/80) of patients and 25% (10/40) of controls. Statistical analysis demonstrated a significant association between the 95A>T SNP and the risk of developing acromegaly.