‎⁨PREreviews de Breaching the cell-envelope barriers of gram-positive and fungal microbes by a type VI secretion system in Acidovorax citrulli⁩ | ⁨PREreview⁩

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Breaching the cell-envelope barriers of gram-positive and fungal microbes by a type VI secretion system in Acidovorax citrulli

por Tong-Tong Pei, Yumin Kan, Zeng-Hang Wang, Ming-Xuan Tang, Hao Li, Shuangquan Yan, Yang Cui, Hao-Yu Zheng, Han Luo y Tao G. Dong

Publicado: 31 de mayo de 2021
Servidor: bioRxiv
DOI: 10.1101/2021.05.31.446370

Resumen

The type VI secretion system (T6SS) is a double-tubular toxin-injection nanomachine widely found in gram-negative human and plant pathogens. The current model depicts that the T6SS spear-like Hcp tube is powered by the contraction of an outer sheath to drill through the envelope of a neighboring cell, achieving cytosol to cytosol delivery. However, gram-positive bacteria seem to be impenetrable to such T6SS action. Here we report that a plant pathogen Acidovorax citrulli (AC) deploys a highly potent T6SS to kill a range of bacteria including Escherichia coli, Pseudomonas aeruginosa , Bacillus subtilis , and Mycobacterium smegmatis as well as fungal species including Candida albicans and Pichia pastoris . Using bioinformatic and biochemical assays, we identified a group of T6SS effectors and characterized one effector RhsB that is critical for interspecies interaction. We report that RhsB contains a conserved YD-repeat domain and a C-terminal nuclease domain. Toxicity of RhsB was neutralized by its downstream immunity proteins through direct interaction. RhsB was cleaved at the C-terminal end and a catalytic mutation within the internal aspartic protease abolished such cleavage. Collectively, the T6SS of AC displays potent activities to penetrate the cell envelope barriers of gram-positive and fungal species, highlighting the greatly expanded capabilities of T6SS in modulating microbiome compositions in complex environments.

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In this short manuscript by Pei et al., authors describe that a plant pathogen, Acidovorax citrulli, uses a T6SS to compete with Gram-positive bacterial and fungal species. A brief characterization of a new effector (named RhsB) is described, which belongs to the nuclease superfamily PD-(D/E)XK.…

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