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This paper discusses a new method of making calcium imaging analysis more efficient and how it can be viewed as practical. This prereview was made to assess the strengths and weaknesses of this paper.

PROS =>

Introduction

• Nice and strong introduction to calcium imaging; included effectively explaining its significance and recent technological advancements (e.g., miniscopes, GRIN lenses)

Methodology

• Clearly described a major problem with quick consumption of available storage and demonstrated the solution they took in order to improve effectiveness. These problems are often very important but usually ignored in research.

• Clearly described their ability to integrate with important tools.

• Descriptively displayed the research team’s parameter hierarchy to show the priorities of the various parameters.

IMPROVEMENTS=>

Introduction

• List of challenges (1–10) is detailed but may fit better partially in Methods or summarized more briefly in the Introduction.

• Paragraph starting with “Despite the expanding ecosystem…” introduces limitations well but lacks a clear transition from the previous paragraph on open science tools.

• Some sections rely on general statements (for example: “many workflows fall short in practice”); could strengthen with more examples or brief specification.

Methodology

• When describing the real-world problems that the research team aimed to fix, they can be more specific when discussing their solution that they used. They can tie it to simpler examples, so it is easier to understand.

Discussion

• Best to re-iterate the challenges that the pipeline intended to tackle -> Gives better closure to paper

• No major limitations mentioned directly for methodology or general paper -> Best to have a limitations section

References

• What format is referenced in? Organize for specific type of format

Competing interests

The authors declare that they have no competing interests.

Use of Artificial Intelligence (AI)

The authors declare that they did not use generative AI to come up with new ideas for their review.