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The use of medicinal plants to treat various conditions and illnesses represents an attractive alternative to conventional pharmaceutical drugs, harnessing ancestral knowledge from various indigenous communities. Mostly obtained through wild harvesting, these plants often lack comprehensive information on hydroponic production. With the aim to provide valuable insights into this knowledge gap, this study focused on evaluating the growth of milk thistle (Silybum marianum) and thyme (Thymus vulgaris) under greenhouse conditions. Two treatments were implemented: one using hydroponics (with inorganic substrate and nutrient solution) and another as control, employing organic substrate without any solution. The experimental design was completely randomized, with five repetitions per treatment. Statistical figures indicated that hydroponic cultivation significantly promoted growth in fresh and dry weight, as well as height, of both plant species. This finding points towards the potential of hydroponics as an effective production method for medicinal plants such as thyme and milk thistle.