Ethnobiological and ethnomedicinal relevance: The absence of standardized criteria and clear formulation guidelines for animal-derived ingredients in Traditional Chinese Medicine (TCM) dietary therapy restricts their effectiveness in subhealth management.Aim: This study aims to develop a three-dimensional (3D) evaluation-triple-component formulation system to quantify Yin-Yang properties (a core TCM classification describing ingredient thermal characteristics as hot/warm, mild, cool/cold) and standardize compatibility.Materials and Methods: Drawing on Lingnan dietary traditions and more than twenty years of clinical data, a quantification model was constructed, assessing animal-derived ingredients based on their environmental characteristics, metabolic properties, and growth cycles. The efficacy of the standardized formulations was then verified through targeted application according to specific constitutional conditions.Results: Qingyuan chicken demonstrated a significant Yang tendency (+4.2), while deep-sea tuna displayed a Yin tendency (−7.2), aligning with historical records in TCM literature. Clinical application of these formulations in subjects with Yang or Yin deficiencies alleviated symptoms such as cold intolerance and dry mouth, respectively. Animals with Yang tendencies possessed 30%–40% saturated fatty acids, whereas those with Yin tendencies contained 25%–30% omega-3 fatty acids.Conclusions: The proposed system establishes a structured approach for evaluating and formulating animal-derived dietary ingredients. It effectively integrates traditional TCM principles with contemporary ethnobiological and ethnomedicinal practices, enhancing precision and reliability in TCM nutritional therapy.