The textile industry continually seeks environmentally friendly and sustainable practices to reduce its ecological footprint. This study investigates the effects of enzymatic wash treatments on denim fabric using both conventional and sustainable machine processes. Enzymes, known for their eco-friendly characteristics, are employed to enhance the aesthetic appeal of denim while minimizing the environmental impact associated with traditional finishing methods. The research involves the application of various enzymes, including Enzymatic DM8639B, Enzymatic DM8642, on denim fabric. Conventional washing machines, commonly used in the textile industry, are compared with innovative sustainable machines designed to optimize water and energy consumption. The study assesses the effectiveness of enzymatic treatments in terms of Washing, Rubbing, and Saliva, Tensile Strength, crucial factors influencing denim quality. Additionally, the environmental impact of both processes is analyzed, considering water usage, energy consumption, and chemical waste generation. Sustainability metrics such as carbon footprint and water footprint are employed to evaluate the eco-friendliness of each method. The findings aim to provide insights into the feasibility of integrating sustainable machine processes into denim production, offering a potential avenue for reducing the environmental impact of garment finishing. This research contributes to the ongoing efforts to develop sustainable practices within the textile industry, aligning with global initiatives for a more environmentally conscious and responsible approach to manufacturing.