؜⁨اكتب مراجعة على PREreview⁩

Machine Learning-Driven Identification of Serotype-Independent Pneumococcal Vaccine Candidates using samples from Human Infection Challenge Studies

Katerina S. Cheliotis وPatricia Gonzalez-Dias وEsther L. German وAndré N. A. Gonçalves وElena Mitsi وElissavet Nikolaou وSherin Pojar وEliane N. Miyaji وRafaella Tostes وJesús Reiné وAndrea M. Collins وHelder I. Nakaya وStephen B. Gordon وYing-Jie Lu وShaun H. Pennington وAndrew J. Pollard وRichard Malley وSimon P. Jochems وBritta Urban وCarla Solórzano وDaniela M. Ferreira

نُشر: 17 سبتمبر 2025
الخادم: medRxiv
DOI: 10.1101/2025.09.12.25335625

Identifying conserved, immunogenic proteins that confer protection against Streptococcus pneumoniae colonisation could enable development of serotype-independent vaccines.

We analysed baseline samples from 86 healthy adults experimentally challenged with pneumococcal serotypes 6B or 15B to investigate whether immune responses to 75 universally expressed pneumococcal proteins associated with protection against colonisation. We measured serum IgG using a novel Luminex assay and cytokine responses from peripheral blood mononuclear cells following antigen stimulation.

No individual IgG or cytokine marker correlated significantly with protection in univariate analysis. However, machine learning identified IgG responses to PdB, SP1069, and SP0899 as predictive of protection. MCP-1 responses to SP1069 and SP0899, and IL-17 production in response to SP0648-3 also correlated with protection. Elevated baseline IFN-γ, RANTES, and anti-protein IgG correlated with lower colonisation density.

We highlight SP1069 and SP0899 as potential serotype-independent vaccine candidates and demonstrate the utility of machine learning to identify immune correlates of protection.

يمكنك كتابة مراجعة PREreview لـ Machine Learning-Driven Identification of Serotype-Independent Pneumococcal Vaccine Candidates using samples from Human Infection Challenge Studies. مراجعة PREreview هي مراجعة لمسودة بحثية أولية، ويمكن أن تتراوح بين بضع جمل وتقرير مطوّل، على غرار تقرير مراجعة الأقران الذي تنظمه المجلات العلمية.

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