Structured PREreview of SARS-CoV-2 ORF8 modulates lung inflammation and clinical disease progression
- Published
- DOI
- 10.5281/zenodo.8429231
- License
- CC BY 4.0
- Does the introduction explain the objective of the research presented in the preprint?
- Yes
- The introduction first provides context on the background of COVID-19 and the SARS-CoV-2 virus. The authors explain the general functions and characteristics of the ORF8 accessory protein. According to the authors, their previous work on accessory protein deletion viruses for SARS-CoV-2 revealed that an ORF8 deletion virus resulted in more lung inflammation compared to a clinical isolate. The authors explain that the objective of their research is to further investigate and characterize the impact of ORF8 in clinical disease progression.
- Are the methods well-suited for this research?
- Highly appropriate
- The methods are very well suited for this research. The authors infect K18-hACE2 mice with WA-1AORF8, following biosafety approval.
- Are the conclusions supported by the data?
- Highly supported
- The conclusions are strongly supported by the data. The researchers present data that reveals that mice infected with WA-1AORF8 demonstrated increased levels of lung inflammation compared to mice with wildtype virus.
- Are the data presentations, including visualizations, well-suited to represent the data?
- Highly appropriate and clear
- The tables and graphs allow the data from the results to be visualized and support the conclusions made.
- How clearly do the authors discuss, explain, and interpret their findings and potential next steps for the research?
- Very clearly
- Is the preprint likely to advance academic knowledge?
- Highly likely
- The preprint is very likely to generate further studies on the topic of ORF8 in SARS-CoV-2, which is still rather elusive. The findings in this paper also confirm and extend previous research done by the researchers.
- Would it benefit from language editing?
- No
- Would you recommend this preprint to others?
- Yes, it’s of high quality
- Is it ready for attention from an editor, publisher or broader audience?
- Yes, as it is
- The paper presents useful findings on a topic that is not well-studied. The paper provides valuable data and information that would be beneficial to spread to a larger audience. It could also generate further discussions on this topic.
Competing interests
The author declares that they have no competing interests.