PREreview of Antibacterial activity of tamoxifen derivatives against methicillin-resistantStaphylococcus aureus
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Review for : Antibacterial activity of tamoxifen derivatives against methicillin-resistant Staphylococcus aureus
by Aniket Sharma1,# and Nikhil Srivastava1,#
1Department of Animal Science, University of Wyoming, USA
#Contributed equally
1. Line 35: The term "microdilution assay" should be corrected to "microbroth dilution assay."
2. Line 57: The word "isolates" is misspelled.
3. Line 70: A reference is missing.
4. Materials and Methods Section: There are crucial details missing about the isolation procedure and the source of the isolates. Additionally, there is no information about ethical clearance for these clinical samples.
5. Line 235: The correct term should be "Luria-Bertani broth."
6. MIC Controls: The rationale behind using P. aeruginosa as a positive control needs to be provided, information regarding negative control, solvent control for the Minimum Inhibitory Concentration (MIC) assay is missing.
8. MIC Analysis: Information regarding how the MIC results were analyzed, whether spectrophotometrically or dye-based, is missing.
9. Bacterial Growth Curve: The reason for using a 1/200 dilution for making the bacterial growth curve should be explained.
10. Membrane Permeability Assay: The starting concentration of cells is not mentioned.
11. Fluorescence Monitoring: The use of fluorescence monitoring with a Typhoon FLA scanner in the membrane permeability assay should be explained in more detail.
12. Alternative Methods: Consider using spectrophotometry as an alternate method for measuring fluorescence.
13. Negative Control for Membrane Permeability: There should be a negative control in the membrane permeability assay that is expected to show increased fluorescence at MIC levels.
14. MDS Data: Information about the molecular dynamic simulation (MDS) data with derivative 2 is missing.
In general, there are multiple spelling and grammatical errors which should be addressed.
Competing interests
The authors declare that they have no competing interests.