Structured PREreview of Asymmetric tethering by exocystin vitrorequires a Rab GTPase, a v-SNARE and a Sac1-sensitive phosphoinositide lipid

Does the introduction explain the objective of the research presented in the preprint?

Yes

Are the methods well-suited for this research?

Highly appropriate

Are the conclusions supported by the data?

Somewhat supported

It is generalized that the tethering reaction to the plasma membrane need both Rabs and R-SNAREs, while it was study only on representative per each of them such as Sec4 and Sso1.

Are the data presentations, including visualizations, well-suited to represent the data?

Somewhat appropriate and clear

The images from the microscopy need to have higher quality and scale bars. Figure legends are lacking the number of samples analyzed.

How clearly do the authors discuss, explain, and interpret their findings and potential next steps for the research?

Neither clearly nor unclearly

They need to discuss more the results based on similar findings in other model organisms.

Is the preprint likely to advance academic knowledge?

Moderately likely

Would it benefit from language editing?

Yes

It might need more logical flow and development of the paragraph as sometimes the sentences are short.

Would you recommend this preprint to others?

Yes, but it needs to be improved

Is it ready for attention from an editor, publisher or broader audience?

Yes, after minor changes

It might need work regarding the discussion of the results.

Competing interests

The author declares that they have no competing interests.