PREreviews of “Antimicrobial Sensitivity Testing Using the Kirby-Bauer Disk Diffusion Method; Limited Utility in Ugandan Hospitals” | PREreview

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Antimicrobial Sensitivity Testing Using the Kirby-Bauer Disk Diffusion Method; Limited Utility in Ugandan Hospitals

by Ivan Segawa, Kenneth Ssebambulidde, Daniel Kiiza, and Jackson Mukonzo

Posted: October 16, 2020
Server: AfricArXiv Preprints
DOI: 10.31730/osf.io/jh96e

Abstract

Background: Antimicrobial resistance (AMR) is a global health threat responsible for increased healthcare costs and mortality. The World Health Organization (WHO) global action plan on AMR recommends antimicrobial sensitivity testing (AST) and surveillance of antibiotic use to address this threat. We conducted a cross-sectional study to determine the utility of AST in three major referral hospitals in Uganda. Methods: We used abstraction checklists to collect data on AST requests, AST performed, AST turnaround time, and dispensed antibiotics, from in-patient files and laboratory and pharmacy records. Antibiotic data were summarized using proportions. The relationship between dispensed antibiotics and AST antibiotic-disks was analyzed using spearman’s rank correlation and simple linear regression. Results: Of the 607 in-patient files reviewed, AST was requested in 24 (4.0%), and done in 13 (2.1%). All three hospitals used the Kirby-Bauer disk diffusion method for AST, with a median turnaround time of 5 days (IQR 4-8). While the frequently used AST antibiotic-disks were cotrimoxazole (13.0%), ampicillin (11.7%), and ceftriaxone (10.5%), the most dispensed antibiotics were metronidazole (30.3%), amoxicillin (19.6%) and ceftriaxone (14.8%). There was a weak correlation (r=0.313, p=0.120) between dispensed antibiotics and AST antibiotic-disks, and AST performance was not associated with antibiotic consumption. Conclusion: We report an underutilization of AST, inconsistent with the hospital antibiotic consumption, and may be related to the use of the disk diffusion method. We recommend alternative faster and better coordination in the procurement of AST diagnostics in Ugandan hospitals.

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4 Rapid PREreviews

by Mekolle Julius, Mercury Shitindo, Adedoyin Adefisan-Adeoye, and Nicholas Outa

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Aggregated Rapid PREreviews
Question	Yes	Unsure	N/A	No
Are the findings novel?	75%	25%	0%	0%
Are the results likely to lead to future research?	100%	0%	0%	0%
Is sufficient detail provided to allow reproduction of the study?	0%	25%	0%	75%
Are the methods and statistics appropriate for the analysis?	25%	25%	0%	50%
Are the principal conclusions supported by the data and analysis?	50%	50%	0%	0%
Does the manuscript discuss limitations?	100%	0%	0%	0%
Have the authors adequately discussed ethical concerns?	50%	0%	0%	50%
Does the manuscript include new data?	100%	0%	0%	0%
Are the data used in the manuscript available?	25%	75%	0%	0%
Is the code used in the manuscript available?	0%	100%	0%	0%
Would you recommend this manuscript to others?	100%	0%	0%	0%
Do you recommend this manuscript for peer review?	100%	0%	0%	0%

1 PREreview

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PREreview by Adedoyin Adefisan-Adeoye et al.

Authored by Adedoyin Adefisan-Adeoye, Aboubakry DIA, Christine Wamunyima Kanyengo, Paa-Nii Johnson, Mekolle Julius, Johanssen Obanda, Lucy Ochola, Nicholas Outa, Wafaa M. Rashed, and Mercury Shitindo

This full PREreview is the result of a live-streamed preprint journal club organized and hosted by AfricArXiv, Eider Africa, eLife, PREreview, and Training Centre in Communication Africa as part of the Open Peer Reviewers in Africa workshop training.

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