Newborn screening is a public health program designed to detect severe inherited disorders in newborns within the first days of a baby’s life. This study assessed the influence of quality pro-cesses in NBS laboratories through testing NBS panels, including GALT, TSH, G6PD, Leu, Met, Phe, C5, C6, C8, C10, C14, and C14:1, under 4˚C, 20˚C, and -50˚C for two months. Results found that sam-ples can be stored at -50°C to maintain stability for most parameters in the NBS panel. The study shows methionine is recognized for its low stability among amino acids at room temperature and GALT among enzymes under all conditions. Moreover, different storage conditions might apply de-pending on the tested analyte of the NBS panel. Therefore, improving the quality process of the NBS laboratory can prevent false positive results and enable the re-use of samples after a period.