Write a PREreview

One-carbon metabolism enzyme Ahcy is a redox sensor that modulates gene expression to protect against light stress-induced retinal degeneration

by Sarah C. Stanhope, Kratika Singhal, Nicolás M. Morato, Yunfei Feng, Gaoya Meng, Makayla N. Marlin, Claudia C. Kotanko, Madolyn M. Jarrett, Andrew D. Mesecar, Graham R. Cooks, and Vikki M. Weake

Posted: April 27, 2025
Server: bioRxiv
DOI: 10.1101/2025.04.25.650714

One-carbon metabolism influences gene expression by providing methyl units for DNA, RNA, and histone methylation. Robust methylation requires rapid hydrolysis of the methylation by-productS-adenosylhomocysteine (SAH) byS-adenosylhomocysteinase (Ahcy). Here, we show Ahcy is a redox-sensitive enzyme that is inhibited by oxidation of a conserved cysteine, C195,in vitroandin vivo. Transient oxidation of Ahcy is neuroprotective in aDrosophilalight stress model where it results in rapid gene expression changes and protects against retinal degeneration. Thus, redox sensing by the one-carbon metabolic enzyme Ahcy enables rapid changes in gene expression in response to changes in redox homeostasis.

You can write a PREreview of One-carbon metabolism enzyme Ahcy is a redox sensor that modulates gene expression to protect against light stress-induced retinal degeneration. A PREreview is a review of a preprint and can vary from a few sentences to a lengthy report, similar to a journal-organized peer-review report.

Before you start

We will ask you to log in with your ORCID iD. If you don’t have an iD, you can create one.