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Pathogenic fungi rely on the cell wall component, chitin, for critical structural and immunological functions. Here a chitin labeling method to visualize the hyphal pathogenic response was developed. The data show that filamentous fungi,Candida albicans, transportN-acetylglucosamine (NAG) bio-orthogonal probes and incorporate them into the cell wall, indicating the probes utility forin vivostudy of the morphological, pathogenic switch. As yeast reside in complex microenvironments, The data show that the opportunistic microbeC. albicans, has developed processes to utilize surrounding bacterial cell wall fragments to initiate the morphogenic switch. The probes are utilized for visualization of growth patterns of pathogenic fungi, providing insights into novel mechanisms for the development of antifungals. Remodeling chitin in fungi using NAG derivatives will advance yeast pathogenic studies.