PREreviews of “Specific long-term changes in anti-SARS-CoV-2 IgG modifications and antibody functions in mRNA, adenovector, and protein subunit vaccines”

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Specific long-term changes in anti-SARS-CoV-2 IgG modifications and antibody functions in mRNA, adenovector, and protein subunit vaccines

by Sebastian Reinig, Chin Kuo, Chia-Chun Wu, Sheng-Yu Huang, Jau-Song Yu, and Shin-Ru Shih

Posted: June 20, 2023
Server: medRxiv
DOI: 10.1101/2023.06.16.23291455

Abstract

Various vaccine platforms were developed and deployed against the COVID-19 disease. The Fc-mediated functions of IgG antibodies are essential in the adaptive immune response elicited by vaccines. However, the long-term changes of protein subunit vaccines and their combinations with mRNA vaccines are unknown. A total of 272 serum and plasma samples were collected from individuals who received first to third doses of the protein subunit Medigen, the mRNA (BNT), or the adenovector AstraZeneca vaccines. The IgG subclass level was measured using enzyme-linked immunosorbent assay, and Fc-N glycosylation was measured using LC-MS/MS. Antibody-dependent phagocytosis (ADCP) and complement deposition (ADCD) of anti-spike (S) IgG antibodies were measured. IgG1 and 3 reached the highest anti-S IgG subclass level. IgG1, 2, and 4 subclass levels significantly increased in mRNA- and Medigen-vaccinated individuals. Fc-glycosylation was stable, except in female BNT vaccinees, who showed increased bisection and decreased galactosylation. Female BNT vaccinees had a higher anti-S IgG titer than that of males. ADCP declined in all groups. ADCD increased in Medigen-vaccinated individuals after the third dose. Each vaccine produced specific long-term changes in Fc structure and function. This finding is critical when selecting a vaccine platform or combination to achieve the desired immune response.

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PREreview by Rebeccah Lijek

Authored by Rebeccah Lijek

Review of “Sebastian Reinig, Kou-Chin, Chia-Chun Wu, Sheng-Yu Huang, Jau Song Yu, & Shin-ru Shih. (2023). Anti-SARS-CoV-2 IgG profile of protein subunit, adenovector and mRNA vaccines. medRxiv, 2023.06.16.23291455. https://doi.org/10.1101/2023.06.16.23291455”
This review was written collaboratively…

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PREreviews of Specific long-term changes in anti-SARS-CoV-2 IgG modifications and antibody functions in mRNA, adenovector, and protein subunit vaccines

1 PREreview