As the most widespread and practical digital representations of living cells, metabolic network models have become increasingly precise and accurate. By integrating cellular resources and abiotic constraints, the prediction functions were significantly expanded in recent years. However, we found that if unreasonable modeling methods were adopted due to the lack of consideration of biological knowledge, the conflicts between stoichiometric and other constraints, such as thermodynamic feasibility and enzyme resource availability, would lead to distorted predictions. In this work, we investigated a prediction anomaly of EcoETM, a constraints-based metabolic network model, and introduced the idea of enzyme compartmentalization into the analysis process. Through rational combination of reactions, we avoid the false prediction of pathway feasibility caused by the unrealistic assumption of free intermediate metabolites. This allowed us to correct the pathway structures of L-serine and L-tryptophan. Specific analysis explains the application method of EcoETM-like model, demonstrating its potential and value in correcting the prediction results in pathway structure by resolving the conflict between different constraints and incorporating the evolved roles of enzymes as reaction compartments. Notably, this work also reveals the trade-off between product yield and thermodynamic feasibility. Finally, we provide a preliminary comparison of the thermodynamic feasibility of ammonia and glutamine as amino donors, which revealed that the direct utilization of ammonia does not have a decisive impact on the thermodynamic feasibility of the anthranilate pathway. Our work is of great value for the structural improvement of constraints-based models.
