Optimizing Cryo-FIB Lamellas for sub-5Å in situ Structural Biology
De autoria de Sagar Khavnekar, Veronika Vrbovská, Magda Zaoralová, Ron Kelley, Florian Beck, Sven Klumpe, Abhay Kotecha, Jürgen Plitzko e Philipp S. Erdmann
Publicado
Servidor
bioRxiv
DOI
10.1101/2022.06.16.496417
We here present a method based on metallic platinum sputtering that can substantially enhance the quality of subtomogram averages from lamellas and thereby reduce the number of particles needed for high-resolution subtomogram averaging. We provide evidence for the physical background of this improvement and demonstrate its usefulness by producing sub-5Å ribosome averages from yeast.
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