PREreviews of “Induction of C4genes evolved through changes incisallowing integration into ancestral C3gene regulatory networks” | PREreview

Skip to preprint details Skip to PREreviews

Induction of C₄genes evolved through changes incisallowing integration into ancestral C₃gene regulatory networks

by Pallavi Singh, Sean R. Stevenson, Ivan Reyna-Llorens, Gregory Reeves, Tina B. Schreier, and Julian M. Hibberd

Posted: July 4, 2020
Server: bioRxiv
DOI: 10.1101/2020.07.03.186395

Abstract

C₄photosynthesis has evolved independently in over sixty lineages and in so doing repurposed existing enzymes to drive a carbon pump that limits the RuBisCO oxygenation reaction. In all cases, gene expression is modified such that C₄proteins accumulate to levels matching those of the photosynthetic apparatus. To better understand this rewiring of gene expression we undertook RNA- and DNaseI-SEQ on de-etiolating seedlings of C₄Gynandropsis gynandra, which is sister to C₃Arabidopsis. Changes in chloroplast ultrastructure and C₄gene expression were coordinated and rapid. C₃photosynthesis and C₄genes showed similar induction patterns, but C₄genes fromG. gynandrawere more strongly induced than orthologs from Arabidopsis. A gene regulatory network predicted transcription factors operating at the top of the de-etiolation network, including those responding to light, act upstream of C₄genes. Light responsive elements, especially G-, E- and GT-boxes were over-represented in accessible chromatin around C₄genes. Moreover,in vivobinding of many G-, E- and GT-boxes was detected. Overall, the data support a model in which rapid and robust C₄gene expression following light exposure is generated through modifications incisto allow integration into high-level transcriptional networks including those underpinned by conserved light responsive elements.

Read the preprint

1 Rapid PREreview

by Facundo Romani

Where can I fill out a Rapid PREreview?

We’ve recently replaced Rapid PREreviews with Structured PREreviews.

You can write a Structured PREreview now.

We’ll be updating older Rapid PREreviews like these soon.

Aggregated Rapid PREreviews
Question	Yes	Unsure	N/A	No
Are the findings novel?	100%	0%	0%	0%
Are the results likely to lead to future research?	100%	0%	0%	0%
Is sufficient detail provided to allow reproduction of the study?	100%	0%	0%	0%
Are the methods and statistics appropriate for the analysis?	100%	0%	0%	0%
Are the principal conclusions supported by the data and analysis?	0%	100%	0%	0%
Does the manuscript discuss limitations?	100%	0%	0%	0%
Have the authors adequately discussed ethical concerns?	0%	0%	100%	0%
Does the manuscript include new data?	100%	0%	0%	0%
Are the data used in the manuscript available?	100%	0%	0%	0%
Is the code used in the manuscript available?	100%	0%	0%	0%
Would you recommend this manuscript to others?	100%	0%	0%	0%
Do you recommend this manuscript for peer review?	100%	0%	0%	0%

1 PREreview

Write a PREreview

PREreview by Facundo Romani

Authored by Facundo Romani

The manuscript “Induction of C4 genes evolved through changes in cis allowing integration into ancestral C3 gene regulatory networks” by Singh and colleagues represent an important contribution to the field of comparative studies of C3 and C4 photosynthesis. This paper analyses the de-etiolation …

Read the PREreview by Facundo Romani